Biotinylated Human FGL1 Protein (Primary Amine Labeling)
Catalog No: FGL1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Asp64-Asn305 (UniProt accession: Q08830).
Product Details
- Molecular Aliases
- FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand; HP041; LFIRE1
- Protein Length
- Asp64-Asn305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGL1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human LAG3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Background
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene. Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII. Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
References
- Wang, J., Sanmamed, M. F., Datar, I., Su, T. T., Ji, L., Sun, J., Chen, L., Chen, Y., Zhu, G., Yin, W., et al.
- . Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell 176, 334-347.e312.
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