Biotinylated Human FGFR1 beta (IIIc) Protein
Catalog No: FGFR1-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGFR1 beta (IIIc) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Pro156-Thr355 (UniProt accession: P11362-7).
Product Details
- Molecular Aliases
- FGFR-1; BFGFR; bFGF-R-1; FLT-2; N-sam; CD331;; FGFR1; bFGF-R1; FLT2
- Protein Length
- Pro156-Thr355
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.23 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FGFR1 beta (IIIc) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human FGFR1 beta (IIIc), His-Avi tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 73.0ng/ml determined by ELISA (QC Test).
Background
Fibroblast growth factor receptor 1 (FGFR1) transmits signals through the plasma membrane regulating essential cellular processes like division, motility, metabolism, and death. Overexpression of FGFR1 is observed in numerous tumors and thus constitutes an attractive molecular target for selective cancer treatment.
References
- Pozniak M, Sokolowska-Wedzina A, Jastrzebski K, Szymczyk J, Porebska N, Krzyscik MA, Zakrzewska M, Miaczynska M, Otlewski J, Opalinski L. FGFR1 clustering with engineered tetravalent antibody improves the efficiency and modifies the mechanism of receptor internalization. Mol Oncol. 2020 Sep;14(9):1998-2021. doi: 10.1002/1878-0261.12740. Epub 2020 Jul 3. PMID: 32511887; PMCID: PMC7463352.
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