Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein
Catalog No: CD16B-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly17-Ser200 (accession: AAA35881.1).
Product Details
- Molecular Aliases
- CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB
- Protein Length
- Gly17-Ser200
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.81 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Fc gamma RIIIB (NA1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 μM as determined in SPR assay (Biacore T200).
Background
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
References
- Qiu W Q, Bruin D D, Brownstein B H, et al. Organization of the human and mouse low-affinity Fc gamma R genes: duplication and recombination[J]. Science, 248.
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