Biotinylated Human FAP Protein (active dimer)
Catalog No: FAP-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FAP Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Leu26-Asp760 (UniProt accession: Q12884-1).
Product Details
- Molecular Aliases
- FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
- Protein Length
- Leu26-Asp760
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 87.10 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human FAP is greater than 95% as determined by SEC-HPLC.
The purity of Biotinylated Human FAP is greater than 95% and the molecular weight of this protein is around 175-200 kDa as determined by SEC-MALS.
Immobilized Biotinylated Human FAP, His Tag at 2μg/ml (100μl/Well) on streptavidin(5μg/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).
Loaded Anti-FAP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human FAP, His-Avi tag with an affinity constant of 1.29 nM as determined in BLI assay.
Background
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
References
- Zou B, Liu X, Zhang B, et al. The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes. J Cancer. 2018;9(18):3278-3286. Published 2018 Sep 7. doi:10.7150/jca.25775
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