Biotinylated Human EMMPRIN/CD147 Protein
Catalog No: EMMPRIN-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human EMMPRIN/CD147 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala22-His205 (accession: NP_940991.1).
Product Details
- Molecular Aliases
- Basigin; BSG; CD147; 5F7; M6; OK; TCSF
- Protein Length
- Ala22-His205
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.02 kDa. Due to glycosylation, the protein migrates to 31-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human EMMPRIN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human EMMPRIN is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human EMMPRIN, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. (QC Test)
Background
CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.
References
- Guindolet D, Gabison EE. Role of CD147 (EMMPRIN/Basigin) in Tissue Remodeling. Anat Rec (Hoboken). 2020 Jun;303(6):1584-1589. doi: 10.1002/ar.24089. Epub 2019 Mar 15. PMID: 30768865.
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