Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 Protein
Catalog No: GDF8-HB001
- Species
- Human/Cyno/Mouse/Rat
- Expression System
- HEK293
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Asp267-Ser375 (Human/Cynomolgus) / Asp268-Ser376 (Mouse/Rat) (accession: O14793(Human) / Q95J86(Cynomolgus) / O08689(Mouse) / O35312(Rat)).
Product Details
- Molecular Aliases
- Myostatin; GDF8; Mstn; GDF-8; Myostatin; Growth differentiation factor-8; Growth differentiation factor 8
- Protein Length
- Asp267-Ser375
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 14.21 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi tag with the EC50 of 2.5ng/ml determined by ELISA. (QC Test)
Immobilized Human/Cynomolgus Activin RIIA, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.
Background
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
References
- Walker RG, McCoy JC, Czepnik M, Mills MJ, Hagg A, Walton KL, Cotton TR, Hyvönen M, Lee RT, Gregorevic P, Harrison CA, Thompson TB. Molecular characterization of latent GDF8 reveals mechanisms of activation. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5): E866-E875. doi: 10.1073/pnas.1714622115. Epub 2018 Jan 18. PMID: 29348202; PMCID: PMC5798348.
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