Biotinylated Human CXCR6 Nanodisc
Catalog No: CXCR6-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CXCR6 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu342 (UniProt accession: O00574).
Product Details
- Molecular Aliases
- CXC-R6; CXCR-6; CDw186; CD186; BONZO; STRL33; TYMSTR; CXCR6
- Protein Length
- Met1-Leu342
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.2 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human CXCR6 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CXCR6 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)
Biotinylated Human CXCR6 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR6 Antibody, hFc Tag with an affinity constant of 5.96 pM as determined in SPR assay (Biacore S200).
Background
CXCR6 is a receptor for the chemokine CXCL16, which exists as a membrane or soluble form. CXCR6 is a marker for resident memory T (TRM) cells that plays a role in immunosurveillance through their interaction with epithelial cells. The interaction of CXCR6 with CXCL16 expressed at the membrane of certain subpopulations of intratumor dendritic cells (DC) called DC3, ideally positions these CXCR6+ T cells to receive a proliferation signal from IL-15 also presented by DC3.
References
- Mabrouk N, Tran T, Sam I, Pourmir I, Gruel N, Granier C, Pineau J, Gey A, Kobold S, Fabre E, Tartour E. CXCR6 expressing T cells: Functions and role in the control of tumors. Front Immunol. 2022 Oct 12;13:1022136. doi: 10.3389/fimmu.2022.1022136. PMID: 36311728; PMCID: PMC9597613.
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