Biotinylated Human CTGF/CCN2 Protein
Catalog No: CTGF-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln27-Ala349 (UniProt accession: Q5M8T4-1).
Product Details
- Molecular Aliases
- IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839; IBP8
- Protein Length
- Gln27-Ala349
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CTGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CTGF, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Background
Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis. CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
References
- Toda N, Mukoyama M, Yanagita M, Yokoi H. CTGF in kidney fibrosis and glomerulonephritis. Inflamm Regen. 2018 Aug 6;38:14. doi: 10.1186/s41232-018-0070-0. PMID: 30123390; PMCID: PMC6091167.
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