Biotinylated Human Coagulation factor III/Tissue Factor Protein
Catalog No: III-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Coagulation factor III/Tissue Factor Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser33-Glu251 (UniProt accession: P13726-1).
Product Details
- Molecular Aliases
- Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
- Protein Length
- Ser33-Glu251
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Coagulation Factor III, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. (QC Test)
Background
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation. Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
References
- Rao LV. Tissue factor as a tumor procoagulant. Cancer Metastasis Rev. 1992 Nov;11(3-4):249-66. doi: 10.1007/BF01307181. PMID: 1423817.
- Carmeliet P, Collen D. Tissue factor. Int J Biochem Cell Biol. 1998 Jun;30(6):661-7. doi: 10.1016/s1357-2725(97)00121-0. PMID: 9695023.
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