Biotinylated Human Claudin 3 Nanodisc Protein
Catalog No: CLDN3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Biotinylated Human Claudin 3 Nanodisc Protein is a full-length multi-pass membrane protein presented in a Nanodisc format for antibody discovery, binding assays and method development. Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Val220 (accession: O15551).
Product Details
- Molecular Aliases
- Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1
- Protein Length
- Met1-Val220
- Expression System
- HEK293
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.
Background
Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.
References
- Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S, Fromm M. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta. 2010 Nov;1798(11):2048-57. doi: 10.1016/j.bbamem.2010.07.014. Epub 2010 Jul 22. PMID: 20655293.
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