Biotinylated Human Claudin 18.2 VLP
Catalog No: CLDN182-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- Biotin, His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Full length Human Claudin 18.2 VLP is expressed in HEK293 cells. It contains amino acid residues Met1-Val261 (It may have cross reaction with anti-His antibody) (UniProt accession: P56856-2).
Product Details
- Molecular Aliases
- CLDN18; CLDN18.2; Claudin-18.2; Claudin18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
- Protein Length
- Met1-Val261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The target protein has a predicted MW of 29 kDa.
- Purity
- > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in PBS, 300mM L-arginine (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
The purity of Biotinylated Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Claudin18.2 VLP at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test).
Biotinylated Human Claudin18.2 VLP captured on CM5 Chip via Streptavidin can bind Anti-Claudin18.2 Antibody with an affinity constant of 1.28 nM as determined in SPR assay (Biacore T200).
Background
Claudin18(CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants,CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.
References
- Singh P, Toom S, Huang Y. Anti-claudin 18.2 antibody as new targeted therapy for advanced gastric cancer[J]. Journal of Hematology & Oncology, 2017, 10(1):105.
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