Biotinylated Human Claudin 18.2 Nanodisc
Catalog No: CLDN182-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Claudin 18.2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val261 (UniProt accession: P56856-2).
Product Details
- Molecular Aliases
- CLDN18; CLDN18.2; Claudin-18.2; Claudin18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
- Protein Length
- Met1-Val261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.60 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human Claudin 18.2 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 52.2ng/ml determined by ELISA. (QC Test)
Biotinylated Human Claudin 18.2 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin18.2 Antibody with an affinity constant of 5.72 nM as determined in SPR assay (Biacore T200).
Background
Claudin18 (CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.
References
- Singh P, Toom S, Huang Y. Anti-claudin 18.2 antibody as new targeted therapy for advanced gastric cancer[J]. Journal of Hematology & Oncology, 2017, 10(1):105.
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