Biotinylated Human Claudin 1 Nanodisc
Catalog No: CLAUDIN1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Claudin 1 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val211 (UniProt accession: O95832).
Product Details
- Molecular Aliases
- Claudin-1; CLDN1; CLD1; SEMP1
- Protein Length
- Met1-Val211
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.69 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human Claudin 1 Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 1 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA. (QC Test)
Biotinylated Claudin 1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 1 Antibody, hFc Tag with an affinity constant of 99.98 nM as determined in SPR assay (Biacore T200).
Background
Claudin-1 (CLDN1), a major component of tight junction complexes in the epithelium, maintains cellular polarity, and plays a critical role in cell-to-cell communication as well as epithelial cell homeostasis. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1 expression has been proposed as a prognostic marker of patient survival.
References
- Clément CMH, Deffieu MS, Dorobantu CM, Baumert TF, Ayala-Nunez NV, Mély Y, Ronde P, Gaudin R. Characterisation of endogenous Claudin-1 expression, motility and susceptibility to hepatitis C virus in CRISPR knock-in cells. Biol Cell. 2020 May;112(5):140-151. doi: 10.1111/boc.201900085. Epub 2020 Feb 20. PMID: 32034780; PMCID: PMC7613415.
- Chang JW, Seo ST, Im MA, Won HR, Liu L, Oh C, Jin YL, Piao Y, Kim HJ, Kim JT, Jung SN, Koo BS. Claudin-1 mediates progression by regulating EMT through AMPK/TGF-β signaling in head and neck squamous cell carcinoma. Transl Res. 2022 Sep;247:58-78. doi: 10.1016/j.trsl.2022.04.003. Epub 2022 Apr 22. PMID: 35462077.
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