Biotinylated Human CEACAM-8/CD66b Protein
Catalog No: CEACAM8-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CEACAM-8/CD66b Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln35-Ser319 (accession: NP_001807.2).
Product Details
- Molecular Aliases
- CD66b; CEACAM8; CGM6; CD67; NCA-95; NCA95
- Protein Length
- Gln35-Ser319
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CEACAM-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay (Biacore T200).
Background
Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.
References
- Yoon J, Terada A, Kita H. CD66b regulates adhesion and activation of human eosinophils. J Immunol. 2007 Dec 15;179(12):8454-62. doi: 10.4049/jimmunol.179.12.8454. PMID: 18056392.
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