Biotinylated Human CEACAM-6/CD66c Protein
Catalog No: CEACAM6-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CEACAM-6/CD66c Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Lys35-Gly320 (UniProt accession: P40199).
Product Details
- Molecular Aliases
- CEACAM6; CD66c; CEAL; NCA; CEA
- Protein Length
- Lys35-Gly320
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CEACAM-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CEACAM-6 is greater than 95% as determined by SEC-HPLC.
Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Biotinylated Human CEACAM6, His-Avi tag with an affinity constant of 3.81 μM as determined in SPR assay (Biacore T200).
Background
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) belongs to the human carcino-embryonic antigen (CEA) family. Numerous lines of studies have indicated that altered expression of CEACAM6 may have a role in carcinogenesis and development.
References
- Sharma N, Srivastava S, Kern F, et al. CEACAM 6, a novel marker for the diagnosis of Barrett's esophagus. Dis Esophagus. 2017;30(7):1-5. doi:10.1093/dote/dox026
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