Biotinylated Human CEACAM-5/CD66e Protein
Catalog No: CEACAM5-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Lys35-Ala685 (UniProt accession: P06731-1).
Product Details
- Molecular Aliases
- CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392; CEACAM5
- Protein Length
- Lys35-Ala685
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CEACAM-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CEACAM-5 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CEACAM-5, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).
Loaded Biotinylated CEACAM-5, His-Avi tag on Steptavidin-Biosensor can bind Anti-CEACAM5 Antibody, hFc Tag with an affinity constant of 0.34 nM as determined in BLI assay.
Background
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.
References
- Li Q, Li Y, Li J, et al. FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis. Int J Biol Sci. 2018;14(7):726-735. Published 2018 May 12. doi:10.7150/ijbs.24505
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