Biotinylated Human CDH6/Cadherin 6 Protein
Catalog No: CDH6-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CDH6/Cadherin 6 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr19-Ala615 (UniProt accession: P55285-1).
Product Details
- Molecular Aliases
- Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD; Kidney cadherin
- Protein Length
- Thr19-Ala615
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 69.29 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CDH6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CDH6 is greater than 90% as determined by SEC-HPLC.
Immobilized Anti-CDH6 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CDH6, His-Avi tag with the EC50 of 0.96μg/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human CDH6, His-Avi tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 94.4ng/ml determined by ELISA.
Background
Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity.
References
- Zhou W, Santos L, Dimitriadis E. Characterization of the role for cadherin 6 in the regulation of human endometrial receptivity. Reprod Biol Endocrinol. 2020 Jun 29;18(1):66. doi: 10.1186/s12958-020-00624-w. PMID: 32600462; PMCID: PMC7322878.
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