Biotinylated Human CD93/C1q R1 Protein
Catalog No: CD93-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr22-Lys580 (UniProt accession: Q9NPY3).
Product Details
- Molecular Aliases
- C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1
- Protein Length
- Thr22-Lys580
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD93 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD93, His-Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test).
Biotinylated Human CD93, His-Avi tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 μM as determined in SPR assay (Biacore T200).
Background
CD93 has been shown critical roles in inflammatory and immune diseases. CD93 silencing increased IL-6 and TSLP, but not IL-33 levels in culture supernatants. HDM-induced asthma mice showed significant airway hyperresponsiveness and inflammation with Th2 cytokine activation, along with decreased CD93 expression in bronchial epithelial cells and lung homogenates but increased serum CD93 levels.
References
- Park HJ, Oh EY, Han HJ, Park KH, Jeong KY, Park JW, Lee JH. Soluble CD93 in allergic asthma. Sci Rep. 2020 Jan 15;10(1):323. doi: 10.1038/s41598-019-57176-2. PMID: 31941986; PMCID: PMC6962376.
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