Biotinylated Human CD81 Nanodisc
Catalog No: CD81-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD81 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Tyr236 (UniProt accession: P60033).
Product Details
- Molecular Aliases
- TAPA-1; Tspan-28; TAPA1; TSPAN28; CVID6; S5.7
- Protein Length
- Met1-Tyr236
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.9 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human CD81 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD81 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. (QC Test)
Biotinylated Human CD81 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD81 Antibody, hFc Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Background
CD81 (TAPA-1) is a member of the tetraspanin family, a large group of evolutionary conserved membrane proteins containing 4 transmembrane domains. The most distinctive feature of the tetraspanin proteins is the ability to form lateral associations with one another and to cluster dynamically with multiple cell surface receptors. Tetraspanins are capable of recruiting specific intracellular signaling enzymes, including protein kinase C, that is known to be involved in integrin phosphorylation.
References
- Levy S, Todd SC, Maecker HT. CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol. 1998;16:89-109. doi: 10.1146/annurev.immunol.16.1.89. PMID: 9597125.
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