Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling)
Catalog No: CD74-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the N-terminus. It contains amino acid residues Gln73-Met232 (accession: NP_004346.1).
Product Details
- Molecular Aliases
- CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia GAMMA; II
- Protein Length
- Gln73-Met232
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CD74, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. (QC Test)
Background
CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.
References
- Valiño-Rivas L, Baeza-Bermejillo C, Gonzalez-Lafuente L, Sanz AB, Ortiz A, Sanchez-Niño MD. CD74 in Kidney Disease. Front Immunol. 2015 Sep 23;6:483. doi: 10.3389/fimmu.2015.00483. PMID: 26441987; PMCID: PMC4585214.
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