Biotinylated Human CD38 Protein
Catalog No: CD38-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD38 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val43-Ile300 (UniProt accession: P28907-1).
Product Details
- Molecular Aliases
- ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 2.4.99.20; EC 3.2.2.6; ADPRC1; EC2.4.99.20; EC3.2.2.6
- Protein Length
- Val43-Ile300
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 32.50 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD38 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD38 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD38, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.
Background
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.
References
- Alba MatasCéspedes, Vidalcrespo A, Rodriguez V, et al. Daratumumab, a Novel Human Anti-CD38 Monoclonal Antibody for the Treatment of Chronic Lymphocytic Leukemia and B-Cell Non–Hodgkin Lymphoma[J]. Blood, 2012.
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