Biotinylated Human CD27/TNFRSF7 Protein
Catalog No: CD27-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Thr21-Ile192 (UniProt accession: P26842).
Product Details
- Molecular Aliases
- CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
- Protein Length
- Thr21-Ile192
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD27 Ligand (Trimer), His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA (QC Test).
Background
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
References
- Buchan S L, Rogel A, Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy[J]. Blood, 2017:blood-2017-07-741025. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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