Biotinylated Human CD19 Protein
Catalog No: CD19-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD19 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu21-Lys291 (UniProt accession: P15391-1).
Product Details
- Molecular Aliases
- CD19; B4; CVID3; Leu-12; MGC12802; Leu12
- Protein Length
- Glu21-Lys291
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CD19, His-Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD19, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
Background
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
References
- Maude S L, Teachey D T, Porter D L, et al. CD19-targeted chimeric antigen receptor T-cell therapy for acute lymphoblastic leukemia[J]. Blood, 2015, 125(26):4017-23.
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