Biotinylated Human CD133 Nanodisc
Catalog No: CD133-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD133 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-His865 (UniProt accession: O43490-1).
Product Details
- Molecular Aliases
- PROM1; PROML1; MSTP061; Prominin-1; AC133
- Protein Length
- Met1-His865
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 110.1 kDa.
- Endotoxin
- Less than 1EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)
Biotinylated Biotinylated Human CD133 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD133 Antibody, hFc Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Background
Prominin-1 (PROM1), also known as CD133, is expressed in hepatic progenitor cells (HPCs) and cholangiocytes of the fibrotic liver. It is a cell surface biomarker that allows the identification of stem and cancer stem cells from different organs. It is also expressed in several differentiated epithelial and non-epithelial cells.
References
- Thamm K, Šimaitė D, Karbanová J, Bermúdez V, Reichert D, Morgenstern A, Bornhäuser M, Huttner WB, Wilsch-Bräuninger M, Corbeil D. Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic. 2019 Jan;20(1):39-60. doi: 10.1111/tra.12618. Epub 2018 Nov 14. PMID: 30328220.
- Lee H, Yu DM, Bahn MS, Kwon YJ, Um MJ, Yoon SY, Kim KT, Lee MW, Jo SJ, Lee S, Koo SH, Jung KH, Lee JS, Ko YG. Hepatocyte-specific Prominin-1 protects against liver injury-induced fibrosis by stabilizing SMAD7. Exp Mol Med. 2022 Aug;54(8):1277-1289. doi: 10.1038/s12276-022-00831-y. Epub 2022 Aug 29. PMID: 36038590; PMCID: PMC9440255.
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