Biotinylated Human CA125/MUC16 Protein
Catalog No: CA125-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CA125/MUC16 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly12660-Met12923 (UniProt accession: Q8WXI7).
Product Details
- Molecular Aliases
- CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; CA125MUC16
- Protein Length
- Gly12660-Met12923
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 52-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CA125 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human CA125 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human CA125, His Avi tag at 5μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human MSLN, hFc Tag with the EC50 of 0.78μg/ml determined by ELISA. (QC Test)
Background
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
References
- Das S, Batra S K. Understanding the Unique Attributes of MUC16 (CA125): Potential Implications in Targeted Therapy[J]. Cancer Research, 2015:0008-5472. CAN-15-1050.
- Rao T D, Park K J, Smith-Jones P, et al. Novel Monoclonal Antibodies Against the Proximal (Carboxy-Terminal) Portions of MUC16[J]. Applied immunohistochemistry & molecular morphology: AIMM / official publication of the Society for Applied Immunohistochemistry, 2010, 18(5):462-472.
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