Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein
Catalog No: ANGPTL3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser17-Lys219 (UniProt accession: Q9Y5C1).
Product Details
- Molecular Aliases
- ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
- Protein Length
- Ser17-Lys219
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Immobilized Biotinylated Human ANGPTL3, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. (QC Test)
Background
ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
References
- Oike Y, Akao M, Kubota Y, et al. Angiopoietin-like proteins: potential new targets for metabolic syndrome therapy[J]. Trends in Molecular Medicine, 2005, 11(10):473-479.D
Have questions about this product?
Our technical team can help with product selection, application questions and order support.