Biotinylated Human ANGPT2/Angiopoietin-2 Protein
Catalog No: ANGPT2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Lys275-Phe496 (UniProt accession: O15123-1).
Product Details
- Molecular Aliases
- AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
- Protein Length
- Lys275-Phe496
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ANGPT2 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human ANGPT2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 μM as determined in SPR assay (Biacore T200).
Background
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
References
- Wu X, Giobbie-Hurder A, Liao X, et al. Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy[J]. Cancer Immunology Research, 2017, 5(1):17-28. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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