Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (active dimer)
Catalog No: ALPG-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile20-Asp503 (UniProt accession: P10696).
Product Details
- Molecular Aliases
- Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
- Protein Length
- Ile20-Asp503
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.
The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% and the molecular weight of this protein is around 105-125 kDa as determined by SEC-MALS.
Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type), His-Avi tag with the EC50 of 27.8ng/ml determined by ELISA. (QC Test)
Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
References
- Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364. doi: 10.1002/jbmr.3225. Epub 2017 Aug
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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