Biotinylated Cynomolgus Siglec-10 Protein
Catalog No: SIGLEC10-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Cynomolgus Siglec-10 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr17-Asn552 (UniProt accession: A0A2K5WBX8).
Product Details
- Molecular Aliases
- SLG2; SIGLEC10; MGC126774; PRO940
- Protein Length
- Thr17-Asn552
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.71 kDa. Due to glycosylation, the protein migrates to 72-82 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 25mM MES, 150mM NaCl, 0.5M Arginine (pH 5.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Cynomolgus Siglec-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen). Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
References
- Barkal AA, et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.
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