Biotinylated Cynomolgus IFN alpha/beta R1 Protein
Catalog No: IFN-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus IFN alpha/beta R1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala25-Lys437 (UniProt accession: A0A7N9D7J0).
Product Details
- Molecular Aliases
- AVP; IFN-alpha/beta R1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-R-1; CRF2-1; IFRC; IFN-alpha/β R1; IFN-R1; IFN-alpha/b R1
- Protein Length
- Ala25-Lys437
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus IFN alpha/beta R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IFN alpha/beta R1 is greater than 95% as determined by SEC-HPLC.
Loaded Biotinylated Cynomolgus IFN alpha/beta R1, His-Avi tag on Streptavidin-Biosensor can bind Human IFN alpha 1, hFc Tag (Cat. IFN-HM2A1) with an affinity constant of 1.20 nM as determined in BLI assay. (QC Test)
Biotinylated Cynomolgus IFN alpha/beta R1, His-Avi tag captured on CM5 Chip via Streptavidin can bind Human IFN alpha 1, hFc Tag (Cat. IFN-HM2A1) with an affinity constant of 1.16 nM as determined in SPR assay (Biacore T200).
Background
IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.
References
- Teunissen P F A, Boshuizen M C, Hollander M R, et al. MAb therapy against the IFN-α/β receptor subunit 1 stimulates arteriogenesis in a murine hindlimb ischaemia model without enhancing atherosclerotic burden[J]. Cardiovascular Research, 2015, 107(2):255-266.
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