Biotinylated Human ANGPTL7/CDT6 Protein
Catalog No: ANGPTL7-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ANGPTL7/CDT6 Protein is expressed in HEK293 cells with a His and Avi tag at the C-terminus. It contains amino acid residues Gln27-Pro346 (UniProt accession: O43827).
Product Details
- Molecular Aliases
- angiopoietin-like 7; Angiopoietin-like factor; ANGPTL7; AngX; CDT6
- Protein Length
- Gln27-Pro346
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.2 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ANGPTL7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ANGPTL7 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human ANGPTL7, His Avi tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 3.09μg/ml determined by ELISA. (QC Test)
Background
Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha.
References
- Núria Comes, Buie L K K, Teresa Borrás. Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma[J]. Genes to cells: devoted to molecular & cellular mechanisms, 2011, 16(2):243-259.
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