Biotinylated Human E-Cadherin/Cadherin 1 Protein
Catalog No: ECADHERINCADHERIN1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human E-Cadherin/Cadherin 1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp155-Ala709 (UniProt accession: P12830-1).
Product Details
- Molecular Aliases
- Cadherin-1; Cadherin1; E-Cadherin; E Cadherin; Epithelial cadherin; CDH1; CD324; Arc-1; CAD1; ECAD; E-CAD; LCAM; UVO; L-CAM;; Arc1
- Protein Length
- Asp155-Ala709
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.43 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human E-Cadherin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
E-cadherin is the core component of epithelial adherens junctions, essential for tissue development, differentiation, and maintenance. It is also fundamental for tissue barrier formation, a critical function of epithelial tissues.
References
- Daulagala AC, Bridges MC, Kourtidis A. E-cadherin Beyond Structure: A Signaling Hub in Colon Homeostasis and Disease. Int J Mol Sci. 2019 Jun 5;20(11):2756. doi: 10.3390/ijms20112756. PMID: 31195621; PMCID: PMC6600153.
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