Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling)
Catalog No: CD3E-CF001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus of CD3E and CD3D. It contains amino acid residues Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D) (accession: Q95LI5.2(CD3E) & Q95LI8(CD3D)).
Product Details
- Molecular Aliases
- CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
- Protein Length
- Gln22-Asp117
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa and 44-46 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Immobilized Anti-CD3 Antibody, hFc Tag at 2μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA.
Background
T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
References
- Penaranda C, Tang Q, Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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