Biotinylated Human Kallikrein 3/PSA Protein (active form) (Primary Amine Labeling)
Catalog No: PSA-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- Biotin
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Kallikrein 3/PSA Protein (active form) is expressed in HEK293 cells without tag. It contains amino acid residues Ile25-Pro261 (UniProt accession: P07288-1).
Product Details
- Molecular Aliases
- PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS
- Protein Length
- Ile25-Pro261
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 26.08 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 0.05% Brij-35 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Kallikrein 3 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Human Kallikrein 3, No Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. (QC Test)
Background
Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro.
References
- Härkönen HH, Mattsson JM, Määttä JA, Stenman UH, Koistinen H, Matero S, Windshügel B, Poso A, Lahtela-Kakkonen M. The discovery of compounds that stimulate the activity of kallikrein-related peptidase 3 (KLK3). ChemMedChem. 2011 Dec 9;6(12):2170-8. doi: 10.1002/cmdc.201100349. Epub 2011 Sep 27. Erratum in: ChemMedChem. 2011 Dec 9;6(12):2123. PMID: 21953896.
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