Biotinylated Human TNF alpha Protein (active trimer)
Catalog No: TNF-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human TNF alpha Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val77-Leu233 (UniProt accession: P01375).
Product Details
- Molecular Aliases
- APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha;TNF alpha
- Protein Length
- Val77-Leu233
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Human TNF alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNF R2, mFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human TNF alpha, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TNFR1, hFc Tag with the EC50 of 18.6ng/ml determined by ELISA.
Biotinylated Human TNF alpha, His Tag captured on CM5 Chip via Streptavidin can bind Human TNFR2, mFc Tag with an affinity constant of 1.18 nM as determined in SPR assay (Biacore T200).
Background
Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
References
- Danese S, Colombel J F, Peyrin-Biroulet L, et al. Review article: the role of anti-TNF in the management of ulcerative colitis - past, present and future[J]. Alimentary Pharmacology & Therapeutics, 2013, 37(9):855-866.
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