Biotinylated Human TSLP Protein
Catalog No: TSLP-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human TSLP Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Tyr29-Gln159 (UniProt accession: Q969D9-1).
Product Details
- Molecular Aliases
- thymic stromal lymphopoietin; TSLP
- Protein Length
- Tyr29-Gln159
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa and 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human TSLP, His Avi tag at 1μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. (QC Test)
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.
Loaded Biotinylated Human TSLP, His Tag on Anti-His-Biosensor can bind Human TSLPR, hFc Tag with an affinity constant of 1.30 nM as determined in BLI assay.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP, His Tag with an affinity constant of 0.20 nM as determined in BLI assay.
Background
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
References
- Corren J, Parnes J R, Wang L, et al. Tezepelumab in Adults with Uncontrolled Asthma[J]. New England Journal of Medicine, 2017, 377(10):936-946.
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