Biotinylated Human IL-17C Protein
Catalog No: IL17C-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-17C Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues His19-Val197 (UniProt accession: Q9P0M4).
Product Details
- Molecular Aliases
- CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884;IL17CMGC126884
- Protein Length
- His19-Val197
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 50mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-17C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17C is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-17C, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17RE, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA (QC Test).
Background
Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold. IL‑17C is Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G.
References
- Vandeghinste N, Klattig, Jürgen, Jagerschmidt C, et al. Neutralization of IL-17C reduces skin inflammation in mouse models of psoriasis and atopic dermatitis[J]. Journal of Investigative Dermatology, 2018:S0022202X1830126X.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.