Biotinylated Human VEGF R2/KDR Protein
Catalog No: VEGFR2-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala20-Glu764 (UniProt accession: P35968-1).
Product Details
- Molecular Aliases
- CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73;VEGFR21
- Protein Length
- Ala20-Glu764
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human VEGF165, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His-Avi tag with the EC50 of 1.27μg/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human VEGF R2, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30μg/ml.
Background
The vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/flk-1) functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. Transcriptional regulation of the VEGFR-2 is complex and may involve multiple putative upstream regulatory elements including E boxes.
References
- Jackson TA, Taylor HE, Sharma D, Desiderio S, Danoff SK. Vascular endothelial growth factor receptor-2: counter-regulation by the transcription factors, TFII-I and TFII-IRD1. J Biol Chem. 2005 Aug 19;280(33):29856-63. doi: 10.1074/jbc.M500335200. Epub 2005 Jun 7. PMID: 15941713.
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