Biotinylated Human KIR2DL5 Protein
Catalog No: KIR2DL5-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human KIR2DL5 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues His22-His240 (accession: NP_065396).
Product Details
- Molecular Aliases
- KIR2DL5A; CD158F; CD158F1; KIR2DL5; KIR2DL5.1; KIR2DL5.3; KIR2DL5A; KIR2DL5B
- Protein Length
- His22-His240
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 26.3 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human KIR2DL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
A recently developed anti-KIR2DL5 (CD158f) antibody has demonstrated KIR2DL5 expression on the surface of NK and T lymphocytes, making it the last functional KIR identified in the human genome. KIR2DL5 belongs to an ancestral lineage of KIR with Ig-like domains of the D0-D2 type, of which KIR2DL4, an HLA-G receptor, is the only other human member.
References
- Cisneros E, Moraru M, Gómez-Lozano N, López-Botet M, Vilches C. KIR2DL5: An Orphan Inhibitory Receptor Displaying Complex Patterns of Polymorphism and Expression. Front Immunol. 2012 Sep 17;3:289. doi: 10.3389/fimmu.2012.00289. PMID: 23060877; PMCID: PMC3443818.
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