Human E-Selectin/CD62E Protein
Catalog No: CD62E-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human E-Selectin/CD62E Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Trp22-Pro556 (UniProt accession: P16581).
Product Details
- Molecular Aliases
- CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
- Protein Length
- Trp22-Pro556
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human E-selectin is greater than 90% as determined by SEC-HPLC.
Immobilized Human E-Selectin, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
Background
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
References
- Muz B, Bazai H Y, Sekula A, et al. Abstract 5005: Inhibition of E-selectin or E-selectin together with CXCR4 resensitizes multiple myeloma to treatment[J]. Cancer Research, 2017, 77(13 Supplement):5005-5005. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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