Human BCMA/TNFRSF17 Protein
Catalog No: BCMA-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human BCMA/TNFRSF17 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Met1-Ala54 (UniProt accession: Q02223-1).
Product Details
- Molecular Aliases
- CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
- Protein Length
- Met1-Ala54
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BCMA is greater than 95% as determined by SEC-HPLC.
Immobilized Human APRIL Trimer, His Tag (Cat. APR-HM110) at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA (QC Test).
Immobilized Human BAFF Trimer, His Tag (Cat. BAF-HM112) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
Immobilized Human BCMA, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BCMA Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Loaded Human BCMA, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His Tag with an affinity constant of 0.24 nM as determined in BLI assay.
Loaded Human BCMA, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 2.45 nM as determined in BLI assay.
Background
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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