PROVETOP
Request a Quote

Biotinylated Human NKG2C&CD94 Protein

Catalog No: NKG2C-HB001

Species
Human
Expression System
HEK293
Tag
His, Avi

Product overview

Recombinant Biotinylated Human NKG2C&CD94 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus of NKG2C and Flag tag at the N-terminus of CD94. It contains amino acid residues Glu98-Leu231(NKG2C) and Ser34-Ile179(CD94) (accession: P26717(NKG2C) & Q13241-1(CD94)).

Product Details

Molecular Aliases
NKG2C&CD94; NKG2C; CD94
Protein Length
Glu98-Leu231
Expression System
HEK293
Theoretical Molecular Weight
The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State
Lyophilized
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution Advice
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data Display

Bis-Tris PAGE
NKG2C-HB001 Bis-Tris-PAGE-white result

Biotinylated Human NKG2C&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
NKG2C-HB001 SEC-HPLC result

The purity of Biotinylated Human NKG2C&CD94 is greater than 90% as determined by SEC-HPLC.

Background

NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.

References

  1. Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
  2. Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
  3. López-Botet M, Muntasell A, Vilches C. The CD94/NKG2C+ NK-cell subset on the edge of innate and adaptive immunity to human cytomegalovirus infection. Semin Immunol. 2014 Apr;26(2):145-51.

Have questions about this product?

Our technical team can help with product selection, application questions and order support.

Contact Technical Support