Biotinylated Human LILRB5/CD85c/LIR-8 Protein
Catalog No: LILRB5-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human LILRB5/CD85c/LIR-8 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Arg18-His456 (UniProt accession: O75023-1).
Product Details
- Molecular Aliases
- CD85C; LIR-8; LIR8
- Protein Length
- Arg18-His456
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human LILRB5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRB5 is greater than 95% as determined by SEC-HPLC.
Background
LILRB5, also known as CD85c andLIR-8, belongs to a family of transmembrane glycoproteins that negatively regulate immune cell activation. Mature human LIR-8 consists of a 435 amino acid (aa) extracellular domain with four Ig-like domains, a 21 aa transmembrane segment, and a 111 aa cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). LILRB5 may act as receptor for class I MHC antigens.
References
- Hogan L E, Jones D C, Allen R L. Expression of the innate immune receptor LILRB5 on monocytes is associated with mycobacteria exposure[J]. Scientific Reports, 2016, 6(1):21780.
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