Biotinylated Human CD161 Protein
Catalog No: CD161-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD161 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln67-Ser225 (UniProt accession: Q12918-1).
Product Details
- Molecular Aliases
- KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
- Protein Length
- Gln67-Ser225
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 41-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD161 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD161, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. (QC Test)
Loaded Anti-CD161 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human CD161, His-Avi tag with an affinity constant of 9.09 nM as determined in BLI assay (Gator® Prime).
Background
CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
References
- Gilles SR, Yohe SL, Linden MA, Dolan M, Hirsch B, Grzywacz B. CD161 Is Expressed in a Subset of T-Cell Prolymphocytic Leukemia Cases and Is Useful for Disease Follow-up. Am J Clin Pathol. 2019 Sep 9;152(4):471-478. doi: 10.1093/ajcp/aqz060. PMID: 31305900.
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