Biotinylated Cynomolgus CDH17/Cadherin 17 Protein
Catalog No: CDH17-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus CDH17/Cadherin 17 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln23-Thr784 (accession: XP_005563762.2).
Product Details
- Molecular Aliases
- Cadherin-17; BILL-cadherin; LI-cadherin; P130; Cdh17; CDH16; HPT-1; cadherin-16;; HPT1
- Protein Length
- Gln23-Thr784
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 87.39 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus CDH17 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Biotinylated Cynomolgus CDH17, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CDH17 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. (QC Test)
Loaded Anti-CDH17 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus CDH17, His-Avi tag with an affinity constant of 1.95 nM as determined in BLI assay.
Background
Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.
References
- Liu X, Huang Y, Yuan H, Qi X, Manjunath Y, Avella D, Kaifi JT, Miao Y, Li M, Jiang K, Li G. Disruption of oncogenic liver-intestine cadherin (CDH17) drives apoptotic pancreatic cancer death. Cancer Lett. 2019 Jul 10;454:204-214. doi: 10.1016/j.canlet.2019.04.022. Epub 2019 Apr 17. PMID: 31004701.
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