Human IL-3 R Beta/CD131 Protein
Catalog No: CD131-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-3 R Beta/CD131 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr17-Trp443 (UniProt accession: P32927-1).
Product Details
- Molecular Aliases
- CDw131; CD131; CSF2RB; IL3RB; IL5RB; AIC2A; Csfgmrb
- Protein Length
- Thr17-Trp443
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 74.63 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human IL-3 R Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-3 R Beta is greater than 90% as determined by SEC-HPLC.
Human IL-3 R Beta, hFc Tag captured on CM5 Chip via Protein A can bind Human GM-CSF, No Tag with an affinity constant of 6.78 μM as determined in SPR assay (Biacore T200) (QC Test).
Background
Interleukin-3 receptor (IL-3R) is a heterodimer that comprises an IL-3 specific alpha chain (IL-3R alpha) and a common beta chain (beta C) that is shared with the receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5. The beta c subunit does not bind any cytokine by itself but is involved in the formation of high-affinity functional receptors for IL-5 and GM-CSF. These receptors belong to the cytokine receptor superfamily, but they are structurally and functionally more related to each other and thus make up a distinct subfamily.
References
- Stomski FC, Sun Q, Bagley CJ, Woodcock J, Goodall G, Andrews RK, Berndt MC, Lopez AF. Human interleukin-3 (IL-3) induces disulfide-linked IL-3 receptor alpha- and beta-chain heterodimerization, which is required for receptor activation but not high-affinity binding. Mol Cell Biol. 1996 Jun;16(6):3035-46. doi: 10.1128/MCB.16.6.3035. PMID: 8649415; PMCID: PMC231298.
- Hara T, Miyajima A. Function and signal transduction mediated by the interleukin 3 receptor system in hematopoiesis. Stem Cells. 1996 Nov;14(6):605-18. doi: 10.1002/stem.140605. PMID: 8948019.
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