Human IL-1R2/IL-1 RII/CD121b Protein
Catalog No: CD121B-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Phe14-Glu343 (accession: NP_004624.1).
Product Details
- Molecular Aliases
- IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β;IL1RT2; IL1R-beta; IL1RII; IL1R-β;IL-1R-b; IL1R-b
- Protein Length
- Phe14-Glu343
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-1R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA (QC Test).
Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Background
The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface.
References
- Orlando S, Sironi M, Bianchi G, Drummond AH, Boraschi D, Yabes D, Mantovani A. Role of metalloproteases in the release of the IL-1 type II decoy receptor. J Biol Chem. 1997 Dec 12;272(50):31764-9. doi: 10.1074/jbc.272.50.31764. PMID: 9395521.
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