Human LRP-6 Protein
Catalog No: LRP6-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG2a)
- Activity
- Activity verified
Product overview
Recombinant Human LRP-6 Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the C-terminus. It contains amino acid residues Ala20-Pro630 (UniProt accession: O75581-1).
Product Details
- Molecular Aliases
- LRP-6; DCAD2; FLJ90062; FLJ90421;LRP6
- Protein Length
- Ala20-Pro630
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 95.32 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human LRP-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 1.03μg/ml determined by ELISA (QC Test).
Immobilized Human SOST, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 18.6ng/ml determined by ELISA.
Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4μg/ml determined by ELISA.
Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human LRP-6, mFc Tag with an affinity constant of 8.97 nM as determined in BLI assay.
Background
Low-density lipoprotein receptor protein 6 (LRP6) is a Wnt co-receptor with essential functions in the Wnt/β-catenin pathway, and mutations in LRP6 gene are linked to many complex human diseases, including metabolic syndrome, cancer, Alzheimer's disease and osteoporosis. LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.
References
- Ren S, Johnson BG, Kida Y, Ip C, Davidson KC, Lin SL, Kobayashi A, Lang RA, Hadjantonakis AK, Moon RT, Duffield JS. LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-1. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1440-5. doi: 10.1073/pnas.1211179110. Epub 2013 Jan 9. Erratum in: Proc Natl Acad Sci U S A. 2016 Nov 14;: PMID: 23302695; PMCID: PMC3557093.
- Wang ZM, Luo JQ, Xu LY, Zhou HH, Zhang W. Harnessing low-density lipoprotein receptor protein 6 (LRP6) genetic variation and Wnt signaling for innovative diagnostics in complex diseases. Pharmacogenomics J. 2018 May 22;18(3):351-358. doi: 10.1038/tpj.2017.28. Epub 2017 Jul 11. PMID: 28696417.
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