Human CCL24 Protein
Catalog No: CCL24-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CCL24 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Val27-Cys119 (UniProt accession: O00175).
Product Details
- Molecular Aliases
- CK-beta-6; Eotaxin-2; MPIF-2; MPIF2; SCYA24; Ckb-6; CCL24; member 24; CK-β-6; Ckb6; CK-b-6; CK-b6
- Protein Length
- Val27-Cys119
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.5 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CCL24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CCL24 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CCL24, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CCL24 Antibody, hFc Tag with the EC50 of 53.8ng/ml determined by ELISA. (QC Test)
Background
C-C motif chemokine ligand 24 (CCL24) is a chemokine that regulates inflammatory and fibrotic activities through its receptor, C-C motif chemokine receptor (CCR3). CCL24 is a chemokine that regulates inflammation and fibrosis. It was found to be significantly expressed in patients with non-alcoholic steatohepatitis, in whom it regulates profibrotic processes in the liver.
References
- Segal-Salto M, Barashi N, Katav A, Edelshtein V, Aharon A, Hashmueli S, George J, Maor Y, Pinzani M, Haberman D, Hall A, Friedman S, Mor A. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage. JHEP Rep. 2020 Jan 2;2(1):100064. doi: 10.1016/j.jhepr.2019.100064. PMID: 32039405; PMCID: PMC7005554.
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