Biotinylated Human Claudin 3 Nanodisc
Catalog No: CLDN3-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val220 (UniProt accession: O15551).
Product Details
- Molecular Aliases
- Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1
- Protein Length
- Met1-Val220
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.4 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human Claudin 3, His Tag at 2 μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. (QC Test)
Biotinylated Human Claudin 3 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 3 Antibody, hFc Tag with an affinity constant of 24.48 nM as determined in SPR assay (Biacore T200).
Background
Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.
References
- Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S, Fromm M. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta. 2010 Nov;1798(11):2048-57. doi: 10.1016/j.bbamem.2010.07.014. Epub 2010 Jul 22. PMID: 20655293.
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